human liver cancer cell lines sk hep1  (ATCC)

Journal: Biology

Article Title: Identification of Clinical Value and Biological Effects of XIRP2 Mutation in Hepatocellular Carcinoma

doi: 10.3390/biology13080633

Figure Lengend Snippet: XIRP2 mutation increased the resistance of HCC cells to fludarabine and oxaliplatin but increased their sensitivity to WEHI-539 and LCL 161. ( A ) The OncoPredict algorithm was used to analyze the change of drug score of 198 drugs between the HCC tissues with the XIRP2 wildtype and the XIRP2 mutation. ( B ) Drug score of fludarabine between the HCC tissues with the XIRP2 wildtype and the XIRP2 mutation. ( C ) Drug score of oxaliplatin between the HCC tissues with the XIRP2 wildtype and the XIRP2 mutation. ( D ) Drug score of WEHI-539 between the HCC tissues with the XIRP2 wildtype and the XIRP2 mutation. ( E ) Drug score of LCL-161 between the HCC tissues with the XIRP2 wildtype and the XIRP2 mutation. SNU475 harbored the XIRP2 mutation (I827V), while HepG2, Hep3B, Huh7, Huh1, and SK-Hep1 harbored the XIRP2 wildtype. ( F ) CCK-8 assays were used to detect the IC50 of fludarabine in SNU475, HepG2, Hep3B, Huh7, Huh1, and SK-Hep1 in 48 h. ( G ) CCK-8 assays was used to detect the IC50 of oxaliplatin in SNU475, HepG2, Hep3B, Huh7, Huh1, and SK-Hep1 in 48 h. ( H ) CCK-8 assays were used to detect the IC50 of WEHI-539 in SNU475, HepG2, Hep3B, Huh7, Huh1, and SK-Hep1 in 48 h. ( I ) CCK-8 assays were used to detect the IC50 of LCL-161 in SNU475, HepG2, Hep3B, Huh7, Huh1, and SK-Hep1 in 48 h. * represents p < 0.05; ** represents p < 0.01. n = 3. The control group was used for comparison. Data are shown as mean ± SD.

Article Snippet: The HCC cell lines HepG2, Hep3B, HuH7, Huhi, and SK-Hep1 were procured from Procell (Wuhan, China), while the SNU475 cells were obtained from Icellbioscience (Shanghai, China).

Techniques: Mutagenesis, CCK-8 Assay, Control, Comparison

Journal: Biology

Article Title: Identification of Clinical Value and Biological Effects of XIRP2 Mutation in Hepatocellular Carcinoma

doi: 10.3390/biology13080633

Figure Lengend Snippet: The XIRP2 mutation increased the protein stability of XIRP2 protein. ( A ) Expression of XIRP2 mRNA levels in the HCC tissues with the XIRP2 mutation and the XIRP2 wildtype from the TCGA databases. A total of 29 HCC patients with XIRP2-MUT in TCGA cohort (pink dots), while the number of patients with XIRP2-WT was 339 (blue dots). ( B ) Expression of XIRP2 mRNA levels in HCC tissues with the XIRP2 mutation and the XIRP2 wildtype from the ICGC databases. A total of 21 HCC patients with XIRP2-MUT in ICGC cohort (pink dots), while the number of patients with XIRP2-WT was 238 (blue dots). ( C ) Expression of the XIRP2 mRNA levels in SNU475, HepG2, Hep3B, Huh7, Huh1, and SK-Hep1. ( D ) Expression of XIRP2 protein levels in SNU475, HepG2, Hep3B, Huh7, Huh1, and SK-Hep1 cells. ( E , F ) Degradation rate of XIRP2 protein in SNU475 and Huh7 cells. * represents p < 0.05; ** represents p < 0.01. n = 3. The control group was used for comparison. Data are shown as mean ± SD. are Original band for XIRP2 and GAPDH in D, are Original band for XIRP2 of Huh7, GAPDH of Huh7, XIRP2 of SNU475 and GAPDH of SNU475 in E.

Article Snippet: The HCC cell lines HepG2, Hep3B, HuH7, Huhi, and SK-Hep1 were procured from Procell (Wuhan, China), while the SNU475 cells were obtained from Icellbioscience (Shanghai, China).

Techniques: Mutagenesis, Expressing, Control, Comparison